DNA Cloning: A Practical Approach will build into an indispensable four volume set offering a complete guide to major techniques and protocols required by modern molecular biology laboratories. This new massively expanded edition is a complete update of Hames and Glover's original major two volume set - focusing on new techniques.

Contenuti

1.: Techniques for transformation of E.coli
2.: Lambda replacement vectors in the construction of genomic DNA libraries
3.: Procedures for cDNA cloning
4.: Making probes
5.: Using cloned DNAs to analyse gene organization and expression
6.: Choices of single-stranded vectors and their application for in vitro mutagenesis
7.: PCR and its applications
8.: DNA sequencing

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