Swine in the Laboratory: Surgery, Anesthesia, Imaging, and Experimental Techniques, Second Edition

For two decades, Swine in the Laboratory: Surgery, Anesthesia, and Experimental Techniques has been the most respected practical technical guide for medical and veterinary researchers using swine as experimental animals. Extensively updated and expanded since the publication of the second edition in 2007 and now sponsored by the American College of Laboratory Animal Medicine (ACLAM), it continues to be the most authoritative source of scientific and technical information on the use of domestic and miniature swine in research.

Additions to the Third Edition Include:

• New chapters on toxicology, transgenics, cancer models, and necropsy techniques
• An overview of porcine models that were not included in the previous edition
• Updates to chapters on the various body systems
• Contributions from 25 new coauthors, as well as a new coeditor
• Significant expansions to the accompanying downloadable resources, including new normal data on farm and minipig breeds, videos on training swine and surgical procedures, updated anatomical imaging, and a colored histological atlas

Detailed sections on anesthesia, analgesia, and perioperative care present the best current practices for using swine in experimental surgery. Providing a deepened reference of the most current information and best techniques in an increasingly popular field since the publication of the first edition, this book is a vital tool in conducting the best possible research using porcine models.

BIOLOGY, HANDLING, HUSBANDRY, AND ANATOMY
Introduction
Breed Selection
Biology
Taxonomy and Nomenclature
Reproduction
Growth and Development
Nutrition
Hematology
Behavior
Husbandry
Disease Prevention
Restraint and Handling
Administration of Medications and Injections
General Anatomy
Model Selection
References

ANESTHESIA, ANALGESIA, AND PERIOPERATIVE CARE
Introduction
Anesthetic Induction and Animal Prep Room Activities
Endotracheal Intubation
Special Perioperative Considerations
Malignant Hyperthermia
Paralytic Agents
Postoperative Monitoring
Preanesthetic Agents
Injectable Anesthetic Agents
Inhalant Anesthetics
Cardiopulmonary Bypass (CPB)
Regional Low-Flow Perfusion and Neural Protection
Analgesics
Commonly Recommended Protocols
General Physiological Effects of Anesthetics
Euthanasia
References

WOUND CLOSURE AND INTEGUMENT
General Principles and Surgical Preparation
Suture Selection for Wound Closure
Skin Flaps and Grafts
Wound-Healing Models
References

GASTROINTESTINAL PROCEDURES
Surgical Anatomy
General Principles of Abdominal Surgery
Postoperative Care
Gastronomy and Gastrostomy Techniques
Pyloroplasty
Enterotomy and Intestinal Fistulation
Intestinal Infusion Catheter Implantation
Intestinal Anastomosis
Intestinal Diversion or Intestinal Bypass
Colonic Anastomosis and Fistulation
Total Colectomy
Rectal Prolapse
Intestinal and Multivisceral Transplantation
Vagotomy
Intraperitoneal Sepsis, Septic Shock, and Hemorrhagic Shock
References

LIVER AND BILIARY SYSTEM
Surgical Anatomy and General Principles of Surgery
Cholecystectomy
Cannulation or Constriction of the Common Bile Duct
Partial Hepatectomy, Liver Biopsy, and Intrahepatic Cannulation
Liver Transplantation
Hepatic Xenoperfusion
References

PANCREAS AND SPLEEN
Surgical Anatomy and General Principles of Surgery
Pancreatectomy, Islet Cell Ablation, and Diabetes
Segmental Pancreatectomy, Pancreatic Transplant, and Islet Cell Isolation
Pancreatic Duct Ablation, Cannulation, and Pancreatitis
Splenectomy, Splenic Vascular Catheterization, and Splenic Transplantation
References

URINARY SYSTEM AND ADRENAL GLANDS
General Principles of Surgery and Surgical Anatomy
Nephrectomy
Partial Nephrectomy and Intrarenal Surgery
Renal Transplant
Cystotomy and Ureteral Diversion
Urinary Tract Obstruction, Reflux Nephropathy, and Hydronephrosis
Perineal Urethrostomy and Urinary Diversion
Preputial Diverticulum Ablation
Adrenalectomy
References

REPRODUCTIVE SYSTEM
General Principles of Surgery
Castration (Orchiectomy) and Vasectomy
Ovariohysterectomy, Hysterectomy, Ovariectomy, or Tubal Ligation
Cesarean Section (C-Section)
Fetal Surgery and Fetal Catheterization
Prolapse of the Reproductive Tract
Endometriosis
Pelvic Adhesions
Artifical Insemination and Embryo Transfer
Mammectomy
References

CARDIOTHORACIC AND VASCULAR SURGERY/CHRONIC INTRAVASCULAR CATHETERIZATION
General Principles of Cardiothoracic Surgery and Surgical Anatomy
Pulmonary System
Cardiovascular System
Miscellaneous Thoracic Procedures

HEAD AND NECK SURGERY/CENTRAL NERVOUS SYSTEM
General Principles of Surgery and Surgical Anatomy
Dental Procedures and Tusk Trimming
Maxillofacial and Craniotomy Procedures
Ocular Surgery
Pharyngostomy Tubes
Thyroidectomy
Parathyroidectomy
Hypophysectomy
Spinal Cord Ischemia and Hemorrhage
Stroke Models
Cerebrospinal Fluid (CSF) Collection and Epidural Injection

MUSCULOSKELETAL SYSTEM AND ORTHOPEDIC PROCEDURES
General Principles of Surgery and Surgical Anatomy
Amputation of a Digit and Hoof Trimming
Tendon and Ligament Repair
Tail Amputation
Hernia Repair
Surgical Approaches to Bones and Joints
Bone Marrow Aspiration

CARDIOVASCULAR CATHETERIZATION, ELECTROPHYSIOLOGY, AND IMAGING LABORATORY PROCEDURES
Introduction
Peripheral Vascular Access for Cardiovascular Catheters
Anesthetics and Adjunct Drugs
Hemodynamics
Angioplasty Balloon Techniques, Intravascular Device Implantations, and Restenosis
Atherosclerosis
Electrophysiology and Electrocardiography (ECG) and Telemetry
Pacemark Implantation and Endocardial Pacemaker Leads
Conduction System Ablation
Angiography
Echocardiography

ENDOSCOPIC, LAPAROSCOPIC, AND MINIMALLY INVASIVE SURGERY by M.M. Swindle and R.H. Hawes
Introduction
General Considerations
Anatomic Considerations for Flexible Endoscopic Research
Performing Endoscopy
Endoscopic Retrograde Cholangiopancreatography Research (ERCP)
Endoscopic Hemostasis (Gastrointestinal Bleeding)
Colonoscopy
Larynoscopy and Bronchoscopy
Training in Flexible Endoscopy
Laparoscopic, Celioscopic, and Thoracoscopic Surgery
Postoperative Care for Laparoscopic and Endoscopic Procedures
Natural Orifice Translumenal Endoscopic Surgery (NOTES)
Surgical Robotics

XENOTRANSPLANTATION AND TRANSGENIC TECHNOLOGIES
Introduction
Ethical Considerations
Immunologic and Physiological Considerations
Strategies for Development of Transgenic Animals
Regulatory Guidelines
Public Health Considerations
Management and Husbandry Strategies
Other Considerations

TOXICOLOGY by Ove Svendsen
Introduction
Domestic Pigs and Minipigs
Strains of Minipigs
Pharmacokinetics and Metabolism
Repeat Dose Toxicology
Reproductive Toxicology
Juvenile Studies
Regulatory Acceptance
Conclusions for Toxicological Models
Acknowledgments

RADIOBIOLOGY

IMAGING TECHNIQUES: CT, MRI, AND PET SCANNING by Aage Kristian Olsen, Dora Zeidler, Kasper Pedersen, Michael Sorensen, Svend Borup Jensen, and Ole Lajord Munk
Scanners
Anesthesia
Surgery
Monitoring
Scanning Procedures
Technical Information for DVD Images

OSSABAW ISLAND MINIATURE SWINE: CARDIOMETABOLIC SYNDROME ASSESSMENT by Michael Sturek, Mouhamad Alloosh, James Wenzel, James P. Byrd, Jason M. Edwards, Pamela G. Lloyd, Johnathan D. Tune, Keith L. March, Michael A. Miller, Eric A. Mokelke, and I. Lehr Brisbin, Jr.
Introduction
Characterization of the Model
Summary and Conclusions
Acknowledgments

APPENDIX
INDEX

References appear at the end of each chapter.