F berthiaume (14 risultati)
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PAP. Condizione: New. New Book. Shipped from UK. Established seller since 2000.
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Da: PBShop.store UK, Fairford, GLOS, Regno UnitoPBShop.store UK
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Paperback. Condizione: new. Paperback. Deoxyribonucleic acid (DNA) microarrays are widely used for differential expression studies and for detection of virulence genes in pure bacterial cultures. Their use in complex microbial samples, such as soil and wastewater, has been relatively less studied. This report presents the result…s of a systematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater. Parameters such as the method of DNA extraction from the samples, the type of immobilized probe (whether polymerase chain reaction [PCR] amplicons or oligonucleotides), the length and method of immobilization of oligonucleotides, the method of DNA labelling, the combination of PCR amplification with microarray hybridization and the choice of PCR targets have been optimized. Results indicate that the combination of PCR followed by microarray hybridization can detect pathogens in wastewater samples down to a 0.1% detection limit. The use of microarrays for bacterial source tracking gave promising results on human samples, however the probes used in this study only provided signals for general indicators of fecal contamination when used on samples of animal origin. The overall conclusion is that microarray technology has not yet reached the stage of routine use for microbiological analysis of wastewater. This report demonstrates: That the hybridization of total genomic DNA on microarrays has a high detection limit, of the order of 10^7 genomes; That the use of long oligonucleotides or PCR amplicons from 16S rDNA, or cpn60 probes has insufficient specificity to differentiate several important pathogens, especially within the Enterobacteriaceae family; That the use of short oligonucleotide immobilized probes coupled with PCR amplification of conserved genes, such as 16S rDNA, cpn60, or wecE, can detect pathogens in wastewater down to a 0.1% (DNA weight/weight) concentration; and The potential of DNA microarrays in BST, even if further research work remains necessary to achieve this goal. Deoxyribonucleic acid (DNA) microarrays are widely used for differential expression studies and for detection of virulence genes in pure bacterial cultures. Their use in complex microbial samples, such as soil and wastewater, has been relatively less studied. This report presents the results of a systematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater. Parameters such as the method of DNA extraction from the samples, the type of immobilized probe (whether polymerase chain reaction [PCR] amplicons or oligonucleotides), the length and method of immobilization of oligonucleotides, the method of DNA labelling, the combination of PCR amplification with microarray hybridization and the choice of PCR targets have been optimized. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.
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Da: Rarewaves USA, OSWEGO, IL, U.S.A.Rarewaves USA
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Paperback. Condizione: New. Deoxyribonucleic acid (DNA) microarrays are widely used for differential expression studies and for detection of virulence genes in pure bacterial cultures. Their use in complex microbial samples, such as soil and wastewater, has been relatively less studied. This report presents the results of a syst…ematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater. Parameters such as the method of DNA extraction from the samples, the type of immobilized probe (whether polymerase chain reaction [PCR] amplicons or oligonucleotides), the length and method of immobilization of oligonucleotides, the method of DNA labelling, the combination of PCR amplification with microarray hybridization and the choice of PCR targets have been optimized. Results indicate that the combination of PCR followed by microarray hybridization can detect pathogens in wastewater samples down to a 0.1% detection limit. The use of microarrays for bacterial source tracking gave promising results on human samples, however the probes used in this study only provided signals for general indicators of fecal contamination when used on samples of animal origin. The overall conclusion is that microarray technology has not yet reached the stage of routine use for microbiological analysis of wastewater. This report demonstrates: That the hybridization of total genomic DNA on microarrays has a high detection limit, of the order of 10^7 genomes; That the use of long oligonucleotides or PCR amplicons from 16S rDNA, or cpn60 probes has insufficient specificity to differentiate several important pathogens, especially within the Enterobacteriaceae family; That the use of short oligonucleotide immobilized probes coupled with PCR amplification of conserved genes, such as 16S rDNA, cpn60, or wecE, can detect pathogens in wastewater down to a 0.1% (DNA weight/weight) concentration; and The potential of DNA microarrays in BST, even if further research work remains necessary to achieve this goal.
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Da: Kennys Bookshop and Art Galleries Ltd., Galway, GY, IrlandaKennys Bookshop and Art Galleries Ltd.
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Condizione: New. 2004. paperback. . . . . .
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Da: Rarewaves.com USA, London, LONDO, Regno UnitoRarewaves.com USA
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Paperback. Condizione: New. Deoxyribonucleic acid (DNA) microarrays are widely used for differential expression studies and for detection of virulence genes in pure bacterial cultures. Their use in complex microbial samples, such as soil and wastewater, has been relatively less studied. This report presents the results of a syst…ematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater. Parameters such as the method of DNA extraction from the samples, the type of immobilized probe (whether polymerase chain reaction [PCR] amplicons or oligonucleotides), the length and method of immobilization of oligonucleotides, the method of DNA labelling, the combination of PCR amplification with microarray hybridization and the choice of PCR targets have been optimized. Results indicate that the combination of PCR followed by microarray hybridization can detect pathogens in wastewater samples down to a 0.1% detection limit. The use of microarrays for bacterial source tracking gave promising results on human samples, however the probes used in this study only provided signals for general indicators of fecal contamination when used on samples of animal origin. The overall conclusion is that microarray technology has not yet reached the stage of routine use for microbiological analysis of wastewater. This report demonstrates: That the hybridization of total genomic DNA on microarrays has a high detection limit, of the order of 10^7 genomes; That the use of long oligonucleotides or PCR amplicons from 16S rDNA, or cpn60 probes has insufficient specificity to differentiate several important pathogens, especially within the Enterobacteriaceae family; That the use of short oligonucleotide immobilized probes coupled with PCR amplification of conserved genes, such as 16S rDNA, cpn60, or wecE, can detect pathogens in wastewater down to a 0.1% (DNA weight/weight) concentration; and The potential of DNA microarrays in BST, even if further research work remains necessary to achieve this goal.
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Da: Revaluation Books, Exeter, Regno UnitoRevaluation Books
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Paperback. Condizione: Brand New. illustrated edition. 112 pages. 10.75x8.25x0.25 inches. In Stock.
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Da: Kennys Bookstore, Olney, MD, U.S.A.Kennys Bookstore
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Condizione: New. 2004. paperback. . . . . . Books ship from the US and Ireland.
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Paperback. Condizione: New. Deoxyribonucleic acid (DNA) microarrays are widely used for differential expression studies and for detection of virulence genes in pure bacterial cultures. Their use in complex microbial samples, such as soil and wastewater, has been relatively less studied. This report presents the results of a syst…ematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater. Parameters such as the method of DNA extraction from the samples, the type of immobilized probe (whether polymerase chain reaction [PCR] amplicons or oligonucleotides), the length and method of immobilization of oligonucleotides, the method of DNA labelling, the combination of PCR amplification with microarray hybridization and the choice of PCR targets have been optimized. Results indicate that the combination of PCR followed by microarray hybridization can detect pathogens in wastewater samples down to a 0.1% detection limit. The use of microarrays for bacterial source tracking gave promising results on human samples, however the probes used in this study only provided signals for general indicators of fecal contamination when used on samples of animal origin. The overall conclusion is that microarray technology has not yet reached the stage of routine use for microbiological analysis of wastewater. This report demonstrates: That the hybridization of total genomic DNA on microarrays has a high detection limit, of the order of 10^7 genomes; That the use of long oligonucleotides or PCR amplicons from 16S rDNA, or cpn60 probes has insufficient specificity to differentiate several important pathogens, especially within the Enterobacteriaceae family; That the use of short oligonucleotide immobilized probes coupled with PCR amplification of conserved genes, such as 16S rDNA, cpn60, or wecE, can detect pathogens in wastewater down to a 0.1% (DNA weight/weight) concentration; and The potential of DNA microarrays in BST, even if further research work remains necessary to achieve this goal.
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Da: AHA-BUCH GmbH, Einbeck, GermaniaAHA-BUCH GmbH
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Taschenbuch. Condizione: Neu. Neuware - Deoxyribonucleic acid (DNA) microarrays are widely used for differential expression studies and for detection of virulence genes in pure bacterial cultures. Their use in complex microbial samples, such as soil and wastewater, has been relatively less studied. This report presents the resul…ts of a systematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater. Parameters such as the method of DNA extraction from the samples, the type of immobilized probe (whether polymerase chain reaction [PCR] amplicons or oligonucleotides), the length and method of immobilization of oligonucleotides, the method of DNA labelling, the combination of PCR amplification with microarray hybridization and the choice of PCR targets have been optimized.
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Da: Rarewaves.com UK, London, Regno UnitoRarewaves.com UK
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Paperback. Condizione: New. Deoxyribonucleic acid (DNA) microarrays are widely used for differential expression studies and for detection of virulence genes in pure bacterial cultures. Their use in complex microbial samples, such as soil and wastewater, has been relatively less studied. This report presents the results of a syst…ematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater. Parameters such as the method of DNA extraction from the samples, the type of immobilized probe (whether polymerase chain reaction [PCR] amplicons or oligonucleotides), the length and method of immobilization of oligonucleotides, the method of DNA labelling, the combination of PCR amplification with microarray hybridization and the choice of PCR targets have been optimized. Results indicate that the combination of PCR followed by microarray hybridization can detect pathogens in wastewater samples down to a 0.1% detection limit. The use of microarrays for bacterial source tracking gave promising results on human samples, however the probes used in this study only provided signals for general indicators of fecal contamination when used on samples of animal origin. The overall conclusion is that microarray technology has not yet reached the stage of routine use for microbiological analysis of wastewater. This report demonstrates: That the hybridization of total genomic DNA on microarrays has a high detection limit, of the order of 10^7 genomes; That the use of long oligonucleotides or PCR amplicons from 16S rDNA, or cpn60 probes has insufficient specificity to differentiate several important pathogens, especially within the Enterobacteriaceae family; That the use of short oligonucleotide immobilized probes coupled with PCR amplification of conserved genes, such as 16S rDNA, cpn60, or wecE, can detect pathogens in wastewater down to a 0.1% (DNA weight/weight) concentration; and The potential of DNA microarrays in BST, even if further research work remains necessary to achieve this goal.
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Da: AussieBookSeller, Truganina, VIC, AustraliaAussieBookSeller
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Paperback. Condizione: new. Paperback. Deoxyribonucleic acid (DNA) microarrays are widely used for differential expression studies and for detection of virulence genes in pure bacterial cultures. Their use in complex microbial samples, such as soil and wastewater, has been relatively less studied. This report presents the result…s of a systematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater. Parameters such as the method of DNA extraction from the samples, the type of immobilized probe (whether polymerase chain reaction [PCR] amplicons or oligonucleotides), the length and method of immobilization of oligonucleotides, the method of DNA labelling, the combination of PCR amplification with microarray hybridization and the choice of PCR targets have been optimized. Results indicate that the combination of PCR followed by microarray hybridization can detect pathogens in wastewater samples down to a 0.1% detection limit. The use of microarrays for bacterial source tracking gave promising results on human samples, however the probes used in this study only provided signals for general indicators of fecal contamination when used on samples of animal origin. The overall conclusion is that microarray technology has not yet reached the stage of routine use for microbiological analysis of wastewater. This report demonstrates: That the hybridization of total genomic DNA on microarrays has a high detection limit, of the order of 10^7 genomes; That the use of long oligonucleotides or PCR amplicons from 16S rDNA, or cpn60 probes has insufficient specificity to differentiate several important pathogens, especially within the Enterobacteriaceae family; That the use of short oligonucleotide immobilized probes coupled with PCR amplification of conserved genes, such as 16S rDNA, cpn60, or wecE, can detect pathogens in wastewater down to a 0.1% (DNA weight/weight) concentration; and The potential of DNA microarrays in BST, even if further research work remains necessary to achieve this goal. Deoxyribonucleic acid (DNA) microarrays are widely used for differential expression studies and for detection of virulence genes in pure bacterial cultures. Their use in complex microbial samples, such as soil and wastewater, has been relatively less studied. This report presents the results of a systematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater. Parameters such as the method of DNA extraction from the samples, the type of immobilized probe (whether polymerase chain reaction [PCR] amplicons or oligonucleotides), the length and method of immobilization of oligonucleotides, the method of DNA labelling, the combination of PCR amplification with microarray hybridization and the choice of PCR targets have been optimized. Shipping may be from our Sydney, NSW warehouse or from our UK or US warehouse, depending on stock availability.
Journal d'un voyage fait par ordre du Roi dans l'Amérique septentrionale. I (1). II (2). 2 Volumes
Charlevoix, F.-X. de (Édition critique par Pierre Berthiaume)
Editore: Les Presses de l'Université de Montréal, Montréal, 1994
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Da: Librairie Bonheur d'occasion (LILA / ILAB), Montréal, QC, CanadaLibrairie Bonheur d'occasion (LILA / ILAB)
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Aggiungi al carrelloCouvertures rigides. Condizione: Très bon. Jaquettes en très bon état. 1112 pages, pagination continue sur les 2 volumes. Sous étuis. Coll. "Bibliothèque du Nouveau Monde".
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- Print on Demand
Da: moluna, Greven, Germaniamoluna
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EUR 43,98
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Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. What is the center.- The functor evaluation.- An alternative approach to universal algebra.- Variations on Beck s tripleability criterion.- Autonomous categories with models.- Adjunction for enriched categories.- Enr…iched functor categories.- One universe .
