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Hardcover. Condizione: New. Ship out in 2 business day, And Fast shipping, Free Tracking number will be provided after the shipment.Pub Date: 2012 Publisher: Science Press Note: If you are required to book the number is greater than the bookstore inventory you can promptly inform the dispensers Tel 15801427360 Contact qq 794153166 (Send staples bibliography). place in bookstores internal transfer cargo 1-2 days. The OUR Books brand new genuine absolute guarantee. when you sign must seriously view the parcel. satisfaction after receipt books. not satisfied directly refusal. this can save Returns cost and time. the problems caused due to reasons of bookstores all unconditional return policy. Thank you for your visit. Assured orders to ensure that your shopping smooth look forward to your good basic information about the title: transgenic technology: Principles and experimental program (3rd edition of the original) (Navigation Edition) List Price: 118.00 yuan Author: Cartwright (Cartwright EJ) Press: Science Press Publication Date: April 1. 2012 ISBN: 9787030338341 words: Page: Revision: 1 Binding: Hardcover Folio: 16 commodity identification: B007VYFF4E Editor's Choice transgenic technology: Principles and experimental program (the original 3rd Edition ) (Navigation Edition) adhering Springer Molecular Biology series consistent style of clear. easy to use. each chapter includes the introduction of related issues. a list of necessary materials and reagents. experimental operation of specific steps. solutions to common problems and defects circumvent. This practical handbook is a comprehensive production of transgenic animals. at the same time brings together experts in the field in the concrete practice of the process of cumulative experiences and valuable experience for the ongoing transgenic research laboratory and is about to enter the field kicking the tires who is priceless. Abstract No Contents Preface writer of the first part of a multi-modality animal transgenic technology transgenic Drosophila transgenic nematode 3 gene in rats using Tol2 transposon system to produce transgenic zebrafish transgenic Xenopus 5 Using DNA nuclear microinjection turn The second part of mice transgenic cell type-specific transgenic mice 7 design key gene transfer vector DNA into the genome of a successful 8 overexpression transgenic before the nuclear microinjection 9 gene targeting carrier 10 gene capture: knockout fast channel 11 of the mouse embryonic stem cells were cultured for 12 targeting in embryonic stem cells 13 by microinjection of the chimeric mice 14 the aggregation obtained chimeric mice 15 mutant mice operative morula cells 16 for mice genomic modification associated bit-specific recombinase 17 Cre transgenic mice 18 large-scale gene mutation in mice 19 mice breeding and rearing of 20 to maintain and preserve laboratory mutant mice biology methods I: save the mutant strain of mice 21 biological methods for the maintenance and preservation of the laboratory mutant mice II: recovery index of the mutant mouse strains No Digest colophon: Illustration: Summary Transgenesis in Drosophila melanogaster relies upon direct the microinjection of emyos and subsequentcrossing of surviving adults. The necessity of crossing single flies to screen for transgenic events limits therange of useful transgenesis techniques to thosc that have a very high frequency of integration. So thatabout 1 in 10 to 1 in 100 surviving adult flies carry a transgene. Until recently. only random P-elementtransgenesis fulfilled these criteria. However. recent advances have ought homologous recombinationand site-directed integration up to and beyond this level of efficiency. For all transgenesis techniquesin Drosophila melanogaster. microinjection of emyos is the central procedure. This chapter gives adetailed protocol for microinjection. and aims to enable the reader to use it for both site-directed inte-gration and for P-element transgenesis. Key words: Drosophila melanogaster. Emyo. Microinje.…

paperback. Condizione: New. Language:Chinese.Paperback. Pub Date: 2025-05 Pages: 464 Publisher: Posts & Telecom Press This book is a sports nursing guide co-authored by experts from the American Association of Sports Nurses. The book is divided into nine units. The first unit explains the sports nursing profession and management. including basic concepts. development paths. facility planning. and financial management. The second unit explains the basic knowledge of human anatomy and physiology that sports nurses should .…