Humana apr 1998 (19 risultati)

Buch. Condizione: Neu. Neuware - The frequency of reports concemmg the interface of biological reco- tion elements to signal transduction technologies has risen dramatically over the last decade. Because any one of a wide variety of biological recognition elements (e. g. , antibodies, receptors, DNA, microorganisms, or enzymes) can theoretically be interfaced with any one of a wide variety of signal transducers (e. g. , optical, electrochemical, thermal, or acoustic), the potential range of devices and techniques can be bewildering. The purpose of this volume and the previous volume in this series is to provide a basic reference and startmg point for investigators in academics, mdustry, and government to begin or expand their biosensors research. This volume, Methods in Biotechnology vol. 7: Affinity Biosensors: Techniques and Protocols, describes a variety of classical and emerging transduction technologies that have been interfaced to bioaffinity elements (e. g. , antibodies and receptors). Some of the reasons for the expansion in the use of affinity-based biosensors include both advances in signal transduction technologies (e. g. , fiber optics, microelectromcs, and microfabrication) and the availability of bioafflmty elements. More specifically, with respect to biological recognttion elements, commercially and noncommercially produced antibodies directed toward a variety of analytes have become widely available. In addition, te- niques for the purification and stabilization of receptors have also significantly improved. As a result of these recent advances in the field, biosensors research and development projects are being pursued by mvestigators from a wide range of disciplines. …

Buch. Condizione: Neu. Neuware - Cell membranes are not, as once believed, inert structures designed to contain the cell contents, but are in fact dynamic structures that are as me- bolically active as the cytosol and other cellular compartments they surround. Thus membranes not only contain mixtures of lipid and phospholipids, but also many proteins both embedded deeply within the membrane structure itself and also more loosely attached on the membrane surfaces. Though many such proteins have long been known to act as transport proteins, ion channels, hormone receptors, G proteins, cytoskeletal anchorage points, and so on, the major advance of recent years is the increasing understanding that the lipids and phospholipids in the membrane bilayer itself are also metabolized to b- logically active products that can diffuse either in the cytosol or in the m- brane bilayer to control the function of other proteins. Thus the concept of lipid-derived second messengers is now firmly established.…

Buch. Condizione: Neu. Neuware - The enormous advances in molecular biology that have been witnessed in . Not recent years have had major impacts on many areas of the biological sciences least of these has been in the field of clinical bacteriology and infectious disease . Molecular Bacteriology: Protocols and ClinicalApplications aims to provide the reader with an insight into the role that molecular methodology has to play in modern medical bacteriology. The introductory chapter ofMolecular Bacteriology: ProtocolsandCli- cal Applications offers a personal overview by a Consultant Medical Microbio- gist of the impact and future potential offered by molecular methods. The next six chapters comprise detailed protocols for a range of such methods . We believe that the use of these protocols should allow the reader to establish the various methods described in his or her own laboratory. In selecting the methods to be included in this section, we have concentrated on those that, arguably, have greatest current relevance to reference clinical bacteriology laboratories; we have deliberately chosen not to give detailed protocols for certain methods, such as multilocus enzyme electrophoresis that, in our opinion, remain the preserve of specialist la- ratories and that are not currently suited for general use. We feel that the methods included in this section will find increasing use in diagnostic laboratories and that it is important that the concepts, advantages, and limitations of each are th- oughly understood by a wide range of workers in the field . …

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -reason for delaying its study has to do with the question of mathematical maturity. \* No use is made here of trigonometric, logarithmic, or expo nential functions except in occasional optional material indicating how such functions can be handled. A perceptive remark made by George P6lya suggests how we can simultaneously learn mathematics and learn 'about' mathematics-i.e., about the nature of mathematics and how it is developed: If the learning of mathematics reflects to any degree the invention of mathematics, it must have a place for guessing, for plausible inference. The reader will find plenty of opportunity here for guessing. The early chapters go at a gentle pace and invite the reader to enter into the spirit of the investigation. Exercises asking the reader to 'make a guess' should be taken in this spirit-as simply an invitation to speculate about what is the likely truth in a given situation without feeling any pressure to guess 'correctly'. Readers will soon realize that a matter about which they are asked to guess will likely be a topic of serious discussion later on. 428 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -No applied mathematician can be properly trained without some basic un derstanding ofnumerical methods, Le., numerical analysis. And no scientist and engineer should be using a package program for numerical computa tions without understanding the program's purpose and its limitations. This book is an attempt to provide some of the required knowledge and understanding. It is written in a spirit that considers numerical analysis not merely as a tool for solving applied problems but also as a challenging and rewarding part of mathematics. The main goal is to provide insight into numerical analysis rather than merely to provide numerical recipes. The book evolved from the courses on numerical analysis I have taught since 1971 at the University ofGottingen and may be viewed as a successor of an earlier version jointly written with Bruno Brosowski [10] in 1974. It aims at presenting the basic ideas of numerical analysis in a style as concise as possible. Its volume is scaled to a one-yearcourse, i.e., a two-semester course, addressing second-yearstudents at a German university or advanced undergraduate or first-year graduate students at an American university. 344 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Optical network design and modelling is an essential issue for planning and operating networks for the next century. The main issues in optical networking are being widely investigated not only for WDM networks but also for optical TDM and optical packet switching. This book aims to contribute to further progress in optical network architectures, design, operation and management and covers the following topics in detail: OAM functions and layered design of photonic networks; network planning and design; network modelling; analysis and protocols of optical LANs; network availability and performance modelling. This book contains the selected proceedings of the International Working Conference on Optical Network Design and Modelling, sponsored by the International Federation for Information Processing (IFIP) and was held in February 1997, in Vienna, Austria. The valuable book will be essential rading for personnel in computer/communication industries, and academic and research staff in computer science and electrical engineering. 174 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In 1962 Clark and Lyons pioneered the concept of a biosensor. They p- posed immobilizing enzymes at electrochemical detectors to form 'enzyme el- trodes' in order to expand the analyte range of ther base sensor. Smce then, the field of blosensors has greatly expanded. Some of the reasons for the expansion include both advances in signal transduction technologies and the incorporation of different biological sensing elements (Table 1). As a consequence, there are now a bewildering array of permutations of the biological sensing element and signal transducers that can be used to c- struct a biosensor. The purpose of the two volumes of Protocols and Te- niques in Biosensors is to provide a basic reference tool and starting point for use by graduate students, postdoctoral and senior researchers, and technicians m academics, industry, and government research establishments, to enable rapid entry into the field of biosensors. There are a variety of approaches that researchers employ to select a combination of bioaffinity elements and signal transducers. One commonly used approach is to identtfy the compound or compounds of interest; identify the biological molecule that yields an appropriate recognitionlselectivtty and dynamic concentration range for the assay; and choose an assay format and signal transduction technology that will meet the analytical requirements for the proposed application, This volume, Enzyme and Microbial Biosensors: Techniques and Protocols, describes a variety of transduction technologies that have been interfaced to enzymes and microorganisms. 280 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This introduction to caclulus was written for liberal arts students, particularly for those whose principal interest is in the humanities. While the usual topics in calculus are covered, the subject is treated in an historical manner that gives a picture of the nature of mathematics and of the place of mathematics within the liberal arts.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 428 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Much current research in computer science is concerned with two questions: is a program correct And how can we improve a correct program preserving correctness This latter question is known as the refinement of programs and the purpose of this book is to consider these questions in a formal setting. In fact, correctness turns out to be a special case of refinement and so the focus is on refinement. Although a reasonable background knowledge is assumed from mathematics and CS, the book is a self-contained introduction suitable for graduate students and researchers coming to this subject for the first time. There are numerous exercises provided of varying degrees of challenge. 540 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume is intended as an introduction into numerical analysis for students in mathematics, physics, and engineering. Instead of attempting to exhaustively cover all parts of numerical analysis, the goal is to guide the reader towards the basic ideas and general principles by way of considering main and important numerical algorithms. Given the rapid development of numerical methods, a reasonable introduction to numerical analysis has to confine itself to presenting a solid foundation by restricting the presentation to the basic principles and procedures.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 344 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -A Course on Borel sets provides a thorough introduction to Borel sets and measurable selections and acts as a stepping stone to descriptive set theory by presenting important techniques such as universal sets, prewellordering, scales, etc. It is well suited for graduate students exploring areas of mathematics for their research and for mathematicians requiring Borel sets and measurable selections in their work.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 284 pp. Englisch. …

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Authoritative clinicians present up-to-date, concise, and practical advice on the diagnosis and treatment of the most common sleep disorders encountered in general practice. They not only review such problems as obstructive sleep apnea, insomnia, circadian rhythm disorders, and narcolepsy, but they also discuss such important topics as pediatric sleep disorders, the pharmacology of sleep medicines, diagnostic testing and technology, and the use of bright light therapy in sleep disorders and depression. Throughout, the emphasis is on obtaining an appropriate patient history and carefully analyzing the available diagnostic and treatment strategies to determine appropriate therapeutic regimens. 252 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Authoritative clinicians present up-to-date, concise, and practical advice on the diagnosis and treatment of the most common sleep disorders encountered in general practice. They not only review such problems as obstructive sleep apnea, insomnia, circadian rhythm disorders, and narcolepsy, but they also discuss such important topics as pediatric sleep disorders, the pharmacology of sleep medicines, diagnostic testing and technology, and the use of bright light therapy in sleep disorders and depression. Throughout, the emphasis is on obtaining an appropriate patient history and carefully analyzing the available diagnostic and treatment strategies to determine appropriate therapeutic regimens.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 252 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -In 1962 Clark and Lyons pioneered the concept of a biosensor. They p- posed immobilizing enzymes at electrochemical detectors to form ¿enzyme el- trodes¿ in order to expand the analyte range of ther base sensor. Smce then, the field of blosensors has greatly expanded. Some of the reasons for the expansion include both advances in signal transduction technologies and the incorporation of different biological sensing elements (Table 1). As a consequence, there are now a bewildering array of permutations of the biological sensing element and signal transducers that can be used to c- struct a biosensor. The purpose of the two volumes of Protocols and Te- niques in Biosensors is to provide a basic reference tool and starting point for use by graduate students, postdoctoral and senior researchers, and technicians m academics, industry, and government research establishments, to enable rapid entry into the field of biosensors. There are a variety of approaches that researchers employ to select a combination of bioaffinity elements and signal transducers. One commonly used approach is to identtfy the compound or compounds of interest; identify the biological molecule that yields an appropriate recognitionlselectivtty and dynamic concentration range for the assay; and choose an assay format and signal transduction technology that will meet the analytical requirements for the proposed application, This volume, Enzyme and Microbial Biosensors: Techniques and Protocols, describes a variety of transduction technologies that have been interfaced to enzymes and microorganisms.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 280 pp. Englisch. …

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Optical network design and modelling is an essential issue for planning and operating networks for the next century. The main issues in optical networking are being widely investigated not only for WDM networks but also for optical TDM and optical packet switching. This book aims to contribute to further progress in optical network architectures, design, operation and management and covers the following topics in detail: OAM functions and layered design of photonic networks;network planning and design;network modelling;analysis and protocols of optical LANs;network availability and performance modelling.This book contains the selected proceedings of the International Working Conference on Optical Network Design and Modelling, sponsored by the International Federation for Information Processing (IFIP) and was held in February 1997, in Vienna, Austria.The valuable book will be essential rading for personnel in computer/communication industries, and academic and research staff in computer science and electrical engineering.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 174 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Much current research in computer science is concerned with two questions: is a program correct And how can we improve a correct program preserving correctness This latter question is known as the refinement of programs and the purpose of this book is to consider these questions in a formal setting. In fact, correctness turns out to be a special case of refinement and so the focus is on refinement. Although a reasonable background knowledge is assumed from mathematics and CS, the book is a self-contained introduction suitable for graduate students and researchers coming to this subject for the first time. There are numerous exercises provided of varying degrees of challenge.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 540 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Ribonucleic acids are central to cellular and molecular processes and perform vital functions in both structural and functional roles. RNA molecules form the bridge between the stable genetic information contained within DNA and enzymes and proteins that carry out much of the metabolism within the cell. Many of the sites of protein synthesis, the ribosomes within the cell, are composed of these ribonucleic acids as are the tRNA molecules that deliver the amino acid building blocks to the ribosomes. Of all the RNA species, the nucleic acid intermediate, messenger RNA, is a desirable source of material to biologists, since this reflects much of, what ultimately, is translated into enzymes and proteins. In order to determine the qualitative and quantitative changes in mRNA expression, a vast number of molecular biological techniques have been developed. Key molecular methods that provide the means to initially isolate and analyze RNA molecules are the focus of this volume. In putting together this collection of protocols, we have tried to provide techniques that are most applicable and widely used. In particular, there are a number of iso- tion techniques included that have been developed, modified, or adapted to enable extraction from a variety of cell types, organisms, or subcellular organelles. Successful isolation of intact RNA is an essential starting point for any sub- quent analysis. This is why we have aimed to make this section comprehensive. The analysis of RNA is the focus of the following chapters. 284 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Ribonucleic acids are central to cellular and molecular processes and perform vital functions in both structural and functional roles. RNA molecules form the bridge between the stable genetic information contained within DNA and enzymes and proteins that carry out much of the metabolism within the cell. Many of the sites of protein synthesis, the ribosomes within the cell, are composed of these ribonucleic acids as are the tRNA molecules that deliver the amino acid building blocks to the ribosomes. Of all the RNA species, the nucleic acid intermediate, messenger RNA, is a desirable source of material to biologists, since this reflects much of, what ultimately, is translated into enzymes and proteins. In order to determine the qualitative and quantitative changes in mRNA expression, a vast number of molecular biological techniques have been developed. Key molecular methods that provide the means to initially isolate and analyze RNA molecules are the focus of this volume. In putting together this collection of protocols, we have tried to provide techniques that are most applicable and widely used. In particular, there are a number of iso- tion techniques included that have been developed, modified, or adapted to enable extraction from a variety of cell types, organisms, or subcellular organelles. Successful isolation of intact RNA is an essential starting point for any sub- quent analysis. This is why we have aimed to make this section comprehensive. The analysis of RNA is the focus of the following chapters.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 284 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This book is aimed at those using colour image processing or researching new applications or techniques of colour image processing. It has been clear for some time that there is a need for a text dedicated to colour. We foresee a great increase in the use of colour over the coming years, both in research and in industrial and commercial applications. We are sure this book will prove a useful reference text on the subject for practicing engineers and scientists, for researchers, and for students at doctoral and, perhaps masters, level. It is not intended as an introductory text on image processing, rather it assumes that the reader is already familiar with basic image processing concepts such as image representation in digital form, linear and non-linear filtering, trans forms, edge detection and segmentation, and so on, and has some experience with using, at the least, monochrome equipment. There are many books cov ering these topics and some of them are referenced in the text, where appro priate. The book covers a restricted, but nevertheless, a very important, subset of image processing concerned with natural colour (that is colour as per ceived by the human visual system). This is an important field because it shares much technology and basic theory with colour television and video equipment, the market for which is worldwide and very large; and with the growing field of multimedia, including the use of colour images on the Inter net.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 484 pp. Englisch.…