springer us aug 2023 (28 risultati)

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 196 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 288 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details aspects and applications of interfacing capillary electrophoresis (CE) with mass spectrometry (MS). Chapters guide readers through approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, as well as various capillary coatings, and a broad range of applications including several top-down and bottom-up proteomic approaches. Additionally, a list of analyte targets was provided consisting of amphetamines, antibiotics, carbohydrates (including glycosaminoglycans and glycopeptides), enantiomers, extracellular matrix metabolites, monoclonal antibodies, and nanoparticles, and therefore covers numerous fields of applications such as pharmaceutical, biomedical, food, agrochemical, and environmental analysis. Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge,Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols aims to provide highly valuable information for both beginners and experts in the field be it students, technical staff, and scientists. 272 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 436 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume introduces different methods to assess the structure and folding of bacterial amyloids and analyze their functions, either in vitro or in vivo. Despite their initial association with neurodegenerative diseases, there is now increasing evidence of alternative roles for amyloids, with beneficial aspects for cells. In particular, prokaryotes use amyloids as functional assemblies, where they are key players in the cell's physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Bacterial Amyloids: Methods and Protocols is an ideal guide for experts and novices alike to further explore these crucial protein assemblies. 352 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 268 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides a comprehensive collection of experimental protocols for investigating ferroptosis in different systems, including cultured cells, animal models, and human tissues. The techniques covered in this book look at various aspects of ferroptosis ranging from the detection of lipid peroxidation to the measurement of glutathione peroxidase activity and the evaluation of mitochondrial morphology. Chapters also discuss basic molecular biology methods such as quantitative PCR and immunoblotting, and advanced imaging techniques such as transmission electron microscopy and confocal fluorescence microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Ferroptosis: Methods and Protocols is a valuable resource for researchers who are interested in studying ferroptosis in different contexts including basic research, drug discovery, and clinical applications. 268 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition provides a sound basis for the molecular investigation of NLR function in health and disease. Chapters focus on NLR as innate immune receptors, 'atypical' inflammasomes, biochemical and novel bioluminescence techniques for the measurement of IL-1b as readout of NLR-based inflammasomes, bioluminescent probe, biochemical and microscopy techniques, techniques to measure caspase-1 activation, cell free systems for the study of inflammasome function, and inflammasome activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, NLR Proteins: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researcher sand experts looking to expand their knowledge. 312 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents a collection of versatile methodologies to investigate prokaryotic gene regulation, with focus on the different levels of information processing and usefulness for various model organisms, whether archaeal, bacterial, or both. The chapters in this book are divided into four sections. Section One covers methods that enable the study of the structure of the bacterial/archaeal chromosome, the main template for all gene regulatory processes, and its epigenetic modification. Section Two looks at a selection of approaches that enable higher levels of understanding of transcription initiation, a key step in information processing. Section Three discusses the investigation of regulating transcription factors, which are often considered the main players in gene regulation in prokaryotic cells. The Fourth Section focuses on the next stage of information processing at which gene regulation occurs, namely the RNA-based level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Prokaryotic Gene Regulation: Methods and Protocols is a valuable resource for researchers interested in learning more about this diverse field. 328 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume discusses the latest techniques used in the diverse fields of single cell 'omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical amplification to mass spectrometry and nanotechnology for the analysis of the chemical constituents of cells. In the Neuromethods series style, chapters include both in-depth overviews, as well as detailed protocols that provide the key advice from specialists you need to get successful results in your laboratory.Cutting-edge and comprehensive, Single Cell 'Omics of Neuronal Cells is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments. 308 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition volume expands on the previous edition with discussions on the latest techniques used to study synaptic transmissions. The chapters in this book are organized into six parts. Part One looks at the basic concepts, such as extracellular and intracellular recordings, and spatiotemporal effects of synaptic currents. Part Two describes the recording of synaptic currents, such as measuring kinetics of synaptic current and measuring reversal potentials. Part Three discusses basic experimentations of synaptic transmission and covers run-up and run-down, and amplitude. Parts Four and Five cover experimentations with computational components and molecular and visual components, such as measurement of a single synapse and electrophysiological and visual tags. Part Six explores in vivo recordings and talks about general considerations for in vivo exploration of synaptic plasticity. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Authoritative and thorough, Electrophysiological Analysis of Synaptic Transmission, Second Edition is a valuable resource that introduces graduate students and postdoctoral fellows to important topics in this field and also expands these topics to practical electrophysiological approaches. 308 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This protocol book presents reproducible and step-by-step procedures for the peptide synthesis, their characterization and applications. The volume includes an introductory section on in silico modelling of new peptide molecules, Molecular Dynamics Simulations, Docking, Electrostatic fingerprinting of peptides, and other modelling tools for peptide designing and optimization. Further, it covers protocols for the solid phase peptide synthesis, chromatographic and mass spectrometric characterization of peptides. Importantly, it covers methods for biophysical characterizations of peptides for their potential applications as drug delivery vehicles, peptide nano-assembly, bionanocatalysis, protein aggregation diseases, and peptide-based anti-bacterial. 156 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This collection provides methods and techniques employed when culturing, manipulating, quantifying, and functionally assessing retinal ganglion cells (RGCs), vital for better understanding various ocular diseases and for developing novel therapies. The book features exhaustive and detailed protocols for the study of RGCs at both the in vitro and in vivo level, including the culture of rodent and human cells, and immunohistochemical, morphological, and functional assessments of various in vivo models of RGC death. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Retinal Ganglion Cells: Methods and Protocols serves as an ideal guide to these important cellular features of the central nervous system and the many traumatic and degenerative blinding diseases that affect them. 208 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition provides a sound basis for the molecular investigation of NLR function in health and disease. Chapters focus on NLR as innate immune receptors, 'atypical' inflammasomes, biochemical and novel bioluminescence techniques for the measurement of IL-1b as readout of NLR-based inflammasomes, bioluminescent probe, biochemical and microscopy techniques, techniques to measure caspase-1 activation, cell free systems for the study of inflammasome function, and inflammasome activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, NLR Proteins: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researcher sand experts looking to expand their knowledge. 312 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents both theoretical guidance and protocols on chemogenomics including chemogenomics library assembly, compound profiling, and phenotypic assays. The chapters in this book cover topics such as the assembly and use of Kinase Chemogenomics; data mining for chemogenomic compound candidates; protocols for protein family-focused assay systems to profile chemogenomic compounds; functional and target engagement assays in cellular settings for broad characterization; and a discussion on phenotypic assays where chemogenomic sets may be applied. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Chemogenomics: Methods and Protocols is a valuable resource for all researchers who are interested in learning more about this diverse and developing field. 248 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering procedures to detect and quantify the damaged bases, protein DNA crosslinks, and double-strand breaks, experimental procedures to identify DNA repair protein interactome by conventional tandem affinity purification followed by mass spectroscopy analysis, as well as the analysis of genome-wide binding of DNA repairproteinsand copy number variations of the DNA damage response gene in tumors. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Base Excision Repair Pathway: Methods and Protocols serves as a valuable resource for novices and experts trying to examine the repair of various types of DNA lesions in vitro and in cell by the distinct set of proteins in the BER pathway. 276 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge,PCR: Methods and Protocols, Second Edition aims to be auseful and practical guide to new researchers and experts looking to expand their knowledge. 268 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides a comprehensive collection of experimental protocols for investigating ferroptosis in different systems, including cultured cells, animal models, and human tissues. The techniques covered in this book look at various aspects of ferroptosis ranging from the detection of lipid peroxidation to the measurement of glutathione peroxidase activity and the evaluation of mitochondrial morphology. Chapters also discuss basic molecular biology methods such as quantitative PCR and immunoblotting, and advanced imaging techniques such as transmission electron microscopy and confocal fluorescence microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Ferroptosis: Methods and Protocols is a valuable resource for researchers who are interested in studying ferroptosis in different contexts including basic research, drug discovery, and clinical applications. 268 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides a comprehensive introduction into methods and procedures on the preparation and characterization of animal tissue-derived extracellular matrix scaffolds primarily from bubaline, caprine, porcine, ovine, rabbit, rat and fish source. Chapters guide readers through decellularization protocol unique to the particular animal tissue and animal studies outcomes.Authoritative and cutting-edge,Tissue Scaffolds aims to be auseful and practical guide to new researchers and experts looking to expand their knowledge. 540 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides methods on the role of TLRs in immune responses and on the various approaches targeting different TLRs in cancer, autoimmunity and in vaccine development. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Toll-Like Receptors: Methods and Protocols aims to be auseful and practical guide to new researchers and experts looking to expand their knowledge. 300 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details protocols for computer-assisted design and experimental characterization of RNA nanostructures. Chapters guide readers through RNA nanotechnology, design and characterization of RNA nanostructures, assessment of immunology of nanomaterials, biosensing and drug delivery, various biomedical applications, and delivery approaches for therapeutic RNA nanoparticles. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, RNA Nanostructures: Design, Characterization, and Applications aims to address essential topics and concerns in the growing field of RNA nanotechnology. 348 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition volume expands on the previous edition with new discussions on the latest techniques used to study molecular chaperones and the stress response. The chapters in this book cover such as analysis of the initiation and regulation of the stress response; the role of heat shock protein 90 (Hsp90) in gene expression through chromosome-immunoprecipitation; features of chaperone function and biology; the emerging role of the extracellular HSPs; and the use of chaperones as biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Chaperones: Methods and Protocols, Second Edition is a valuable resource for all researchers who want to learn more about this interesting and developing field. 344 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume looks at the latest methods used to study imaging techniques, metabolic tracing, and deep muscle phenotyping. The chapters in this book cover topics such as imaging skeletal muscle mass using MRI, dual-energy x-ray absorptiometry, CT, and ultrasound; measures of neuromuscular function such as power/strength/force; microscopy and immunohistochemistry; and ex vivo fibre function and isolation. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and thorough, Neuromuscular Assessments of Form and Function is a valuable resource for researchers interested in multiple methods used to study skeletal muscle neurophysiology. 332 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition details new and updated protocols for experimental approaches that are currently used to study the formation of flowers. Chapters guide readers on genetic methods, phenotypic analyses, genome-wide experiments, modeling, and system-wide approaches. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Flower Development: Methods and Protocols, Second Editionaimsto be auseful and practical guide to new researchersand experts looking to expand their knowledge. 604 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Language, as a system we use to communicate, represents the brain's biologically perfected machinery for converting thoughts (ideas, concepts, and reflections of both the outside world and our inner feelings) into words and sentences. Crucially, this process occurs in real time. How hundreds of billions of neurons within the dark of the skull control language and speech remains, in some respects, a mystery. To track such neural dynamics in time, we need to exploit physiological tools capable of following temporal patterns of neural activity on a fine-grain time scale. In parallel, it is necessary to begin to provide a real interdisciplinary academic background for scholars wishing to embark on this field of study. Unlike many similar efforts, this book has been conceived as a hands-on tool offering the reader the possibility to progressively acquire principles, techniques, and methods necessary to pursue interdisciplinary research in a fascinating field intersecting linguistic and neuroscience. It focuses on neurophysiological methods and applications useful to track the high speed and rapid temporal dynamics of neural activity involved in language and speech. The chapters in this book are organized into four parts. Part One discusses neural principles and tools for an effective approach to the field of investigation. Part Two looks at the issues and perspectives concerned with the use of a range of neurophysiological technologies to investigate the neural computations of language and speech processes. Part Three focuses on an in-depth exploration of the neural processes associated with the main types of linguistic information, ranging from phonemes and prosody to syntax, pragmatics, and figurative language. Lastly, Part Five explores the phenomena that goes beyond the segments of basic linguistic units. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory Cutting-edge and thorough, Language Electrified: Principles, Methods, and Future Perspectives of Investigation is a valuable resource that offers the necessary tool-box for all researchers and scientists interested in the challenging field of the neurophysiology of language and speech. 820 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume in the Encyclopedia of Complexity and Systems Science, Second Edition, covers recent developments in classical areas of ergodic theory, including the asymptotic properties of measurable dynamical systems, spectral theory, entropy, ergodic theorems, joinings, isomorphism theory, recurrence, nonsingular systems. It enlightens connections of ergodic theory with symbolic dynamics, topological dynamics, smooth dynamics, combinatorics, number theory, pressure and equilibrium states, fractal geometry, chaos. In addition, the new edition includes dynamical systems of probabilistic origin, ergodic aspects of Sarnak's conjecture, translation flows on translation surfaces, complexity and classification of measurable systems, operator approach to asymptotic properties, interplay with operator algebras 716 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Language, as a system we use to communicate, represents the brain¿s biologically perfected machinery for converting thoughts (ideas, concepts, and reflections of both the outside world and our inner feelings) into words and sentences. Crucially, this process occurs in real time. How hundreds of billions of neurons within the dark of the skull control language and speech remains, in some respects, a mystery. To track such neural dynamics in time, we need to exploit physiological tools capable of following temporal patterns of neural activity on a fine-grain time scale. In parallel, it is necessary to begin to provide a real interdisciplinary academic background for scholars wishing to embark on this field of study. Unlike many similar efforts, this book has been conceived as a hands-on tool offering the reader the possibility to progressively acquire principles, techniques, and methods necessary to pursue interdisciplinary research in a fascinating field intersecting linguistic andneuroscience. It focuses on neurophysiological methods and applications useful to track the high speed and rapid temporal dynamics of neural activity involved in language and speech. The chapters in this book are organized into four parts. Part One discusses neural principles and tools for an effective approach to the field of investigation. Part Two looks at the issues and perspectives concerned with the use of a range of neurophysiological technologies to investigate the neural computations of language and speech processes. Part Three focuses on an in-depth exploration of the neural processes associated with the main types of linguistic information, ranging from phonemes and prosody to syntax, pragmatics, and figurative language. Lastly, Part Five explores the phenomena that goes beyond the segments of basic linguistic units. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratorySpringer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 820 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume in the Encyclopedia of Complexity and Systems Science, Second Edition, covers recent developments in classical areas of ergodic theory, including the asymptotic properties of measurable dynamical systems, spectral theory, entropy, ergodic theorems, joinings, isomorphism theory, recurrence, nonsingular systems. It enlightens connections of ergodic theory with symbolic dynamics, topological dynamics, smooth dynamics, combinatorics, number theory, pressure and equilibrium states, fractal geometry, chaos. In addition, the new edition includes dynamical systems of probabilistic origin, ergodic aspects of Sarnak's conjecture, translation flows on translation surfaces, complexity and classification of measurable systems, operator approach to asymptotic properties, interplay with operator algebrasSpringer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 716 pp. Englisch.