ISBN: 9780896030909 - methods of protein microcharacterization: a practical handbook (17 risultati)

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Chapters written by experts in the new (1986) instrumentation and techniques to sequence peptides and amino acids, edited by John Shively. -- Hardcover. Condition: very good, with very good dust jacket. ISBN 0896030903.

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Condizione: New. Series: Methods in Molecular Biology. Num Pages: 456 pages, biography. BIC Classification: PSBC. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 227 x 152 x 30. Weight in Grams: 872. . 1986. Hardback. . . . .

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Condizione: New. Series: Methods in Molecular Biology. Num Pages: 456 pages, biography. BIC Classification: PSBC. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 227 x 152 x 30. Weight in Grams: 872. . 1986. Hardback. . . . . Books ship from the US and Ireland.…

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Gebunden. Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Microisolation Techniques.- Microisolation of Biologically Active Polypeptides by Reverse-Phase Liquid Chromatography.- Reverse-Phase HPLC Isolation and Microsequence Analysis.- Purification of Microgram Quantities of Proteins by Polyacrylamide Gel Electrop.…

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Buch. Condizione: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role in the determination of polypeptide structures. Important modifications of Edman chemistry include the solid-phase approach by Laursen in 1971 (4) and the use of modified Edman reagents such as 4-N,N-dimethylaminoazobenzene-4'-isothiocy- ate (DABITC) for manual sequencing by Chang et al. (5) in 1976. A second major breakthrough in the analysis of polypeptides was automated amino acid analysis described by Spackman et al. in 1958 (6). However, during the period from 1975 to 1980, it became increasingly clear that the amount of material required for struc tural analysis was more than could be easily isolated for the vast majority of proteins. The field was criticized for its lack of sensitive techniques for the analysis of growth factors, immune modulators, membrane receptors, and peptide hormones. In addition, very little had been done to modernize and improve the original instruments introduced in the mid-1960s. The first indications of improved instrumentation for Edman chemistry came from Wittmann-Liebold's laboratory (7), followed by the introduction of a 'micro' sequencer by Hunkapiller and Hood in 1978 (8). The movement toward improved instrumentation culminated in the 'gas'-phase sequencer of Hewick et al. (9) in 1981.…

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Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role in the determination of polypeptide structures. Important modifications of Edman chemistry include the solid-phase approach by Laursen in 1971 (4) and the use of modified Edman reagents such as 4-N,N-dimethylaminoazobenzene-4'-isothiocy- ate (DABITC) for manual sequencing by Chang et al. (5) in 1976. A second major breakthrough in the analysis of polypeptides was automated amino acid analysis described by Spackman et al. in 1958 (6). However, during the period from 1975 to 1980, it became increasingly clear that the amount of material required for struc tural analysis was more than could be easily isolated for the vast majority of proteins. The field was criticized for its lack of sensitive techniques for the analysis of growth factors, immune modulators, membrane receptors, and peptide hormones. In addition, very little had been done to modernize and improve the original instruments introduced in the mid-1960s. The first indications of improved instrumentation for Edman chemistry came from Wittmann-Liebold's laboratory (7), followed by the introduction of a 'micro' sequencer by Hunkapiller and Hood in 1978 (8). The movement toward improved instrumentation culminated in the 'gas'-phase sequencer of Hewick et al. (9) in 1981.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 480 pp. Englisch.…

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Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role in the determination of polypeptide structures. Important modifications of Edman chemistry include the solid-phase approach by Laursen in 1971 (4) and the use of modified Edman reagents such as 4-N,N-dimethylaminoazobenzene-4'-isothiocy- ate (DABITC) for manual sequencing by Chang et al. (5) in 1976. A second major breakthrough in the analysis of polypeptides was automated amino acid analysis described by Spackman et al. in 1958 (6). However, during the period from 1975 to 1980, it became increasingly clear that the amount of material required for struc tural analysis was more than could be easily isolated for the vast majority of proteins. The field was criticized for its lack of sensitive techniques for the analysis of growth factors, immune modulators, membrane receptors, and peptide hormones. In addition, very little had been done to modernize and improve the original instruments introduced in the mid-1960s. The first indications of improved instrumentation for Edman chemistry came from Wittmann-Liebold's laboratory (7), followed by the introduction of a 'micro' sequencer by Hunkapiller and Hood in 1978 (8). The movement toward improved instrumentation culminated in the 'gas'-phase sequencer of Hewick et al. (9) in 1981. 480 pp. Englisch.…