Isbn: 9783764319250 - a laboratory guide to genomic sequencing: the direct sequencing of native uncloned dna (19 risultati)

Hardback. Condizione: Good. Series: BioMethods. 163p hardback, canary-yellow laminated boards, sunned spine, firm binding, pages clear and bright, many photographs and diagrams, small label to endpaper, small stamps to title page and one or two pages, minimal wear, a good copy from the Cambridge Laboratory of Molecular Biology Language: English Weight (g): 475.…

Condizione: Fair. Volume 1. This is an ex-library book and may have the usual library/used-book markings inside.This book has hardback covers. In fair condition, suitable as a study copy. No dust jacket. Please note the Image in this listing is a stock photo and may not match the covers of the actual item,550grams, ISBN:3764319259.…

Condizione: New.

Paperback or Softback. Condizione: New. A Laboratory Guide to Genomic Sequencing: The Direct Sequencing of Native Uncloned DNA. Book.

Condizione: New.

Condizione: As New. Unread book in perfect condition.

Condizione: New. In.

Condizione: New.

Condizione: New. Series: Biomethods. Num Pages: 164 pages, 31 black & white illustrations, biography. BIC Classification: YQS. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 254 x 178 x 9. Weight in Grams: 475. . 1987. Paperback. . . . . …

Condizione: New. pp. 170.

Condizione: As New. Unread book in perfect condition.

Paperback. Condizione: Brand New. 1st edition. 168 pages. German language. 10.00x7.00x0.40 inches. In Stock.

Condizione: New. Series: Biomethods. Num Pages: 164 pages, 31 black & white illustrations, biography. BIC Classification: YQS. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 254 x 178 x 9. Weight in Grams: 475. . 1987. Paperback. . . . . Books ship from the US and Ireland. …

Taschenbuch. Condizione: Neu. Druck auf Anfrage Neuware - Printed after ordering - A Safety Considerations Genomic sequencing involves a number of hazardous steps, such as high current, high voltage, radioactive and highly toxic chemicals. It is, therefore, absolutelyessen tial that the instructions of equipment manufacturers be followed and that particular attention is paid to the local and federal safety regulations. INTRODUCTION 9 B Introduction During the cloning of genomic DNA many of its characteristics are perma nently lost. It was therefore necessary to develop a new technique that would give us a closer look at a gene in its normal environment. The powerful technique of genomic sequencing, first described by Church and Gilbert (1984) now makes it possible to have a precise view of a given DNA sequence in a chromosome. This method combines the chemical DNA-sequencing procedure of Maxam and Gilbert (1980) with the detection of DNA sequences by electroblotting and indirect end-labeling by hybridization. Besides studies on the methylation state of single bases in a given gene (Nick et al. , 1986; Saluz and Jost, 1986; Saluz et al. , 1986), genomic sequencing can also be used to study specific DNA-protein interactions in vivo (Church et al. , 1985; Giniger et al. , 1985; Becker et al. , 1986; Ephrussi et al. , 1985; Martin et al. , 1986; Nick et al. , 1986; Zinn and Maniatis, 1986).…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -A Safety Considerations Genomic sequencing involves a number of hazardous steps, such as high current, high voltage, radioactive and highly toxic chemicals. It is, therefore, absolutelyessen tial that the instructions of equipment manufacturers be followed and that particular attention is paid to the local and federal safety regulations. INTRODUCTION 9 B Introduction During the cloning of genomic DNA many of its characteristics are perma nently lost. It was therefore necessary to develop a new technique that would give us a closer look at a gene in its normal environment. The powerful technique of genomic sequencing, first described by Church and Gilbert (1984) now makes it possible to have a precise view of a given DNA sequence in a chromosome. This method combines the chemical DNA-sequencing procedure of Maxam and Gilbert (1980) with the detection of DNA sequences by electroblotting and indirect end-labeling by hybridization. Besides studies on the methylation state of single bases in a given gene (Nick et al. , 1986; Saluz and Jost, 1986; Saluz et al. , 1986), genomic sequencing can also be used to study specific DNA-protein interactions in vivo (Church et al. , 1985; Giniger et al. , 1985; Becker et al. , 1986; Ephrussi et al. , 1985; Martin et al. , 1986; Nick et al. , 1986; Zinn and Maniatis, 1986). 168 pp. Englisch.…

Condizione: New. Print on Demand pp. 170 66:B&W 7 x 10 in or 254 x 178 mm Perfect Bound on White w/Gloss Lam.

Condizione: New. PRINT ON DEMAND pp. 170 Acknowledgements.

Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. I. Introduction.- A Safety Considerations.- B Introduction.- C The Principle of Genomic Sequencing.- II Theoretical Background.- A Basic Theoiy of Genomic Sequencing.- B Flow Diagram.- III Experimental.- 1 Isolation of Genomic DNA.- 2 Restriction Digest of . …

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -I. Introduction.- A Safety Considerations.- B Introduction.- C The Principle of Genomic Sequencing.- II Theoretical Background.- A Basic Theoiy of Genomic Sequencing.- B Flow Diagram.- III Experimental.- 1 Isolation of Genomic DNA.- 2 Restriction Digest of Genomic DNA.- 3 Chemical Sequencing Reactions on Restricted DNA.- 4 Separation of Reaction Products on a Sequencing Gel.- 5 Electrotransfer to Nylon Membranes.- 6 Immobilization of DNA on a Nylon Membrane.- 7 Prehybridization and Hybridization of Immobilized DNA with Labeled Single-Stranded DNA Probes.- 8 Processing of the Hybridized Filters.- 9 Autoradiography and Photography.- 10 Cloning of DNA Probe in M13.- 11 Large-Scale Preparation of Cloned DNA in M13.- 12 Synthesis of Oligonucleotide Primers and Single-Stranded Labeled Probes.- 13 Purification of Labeled Single-Stranded Probes.- IV Trouble-Shooting Guide and Examples.- V Appendix.- VI Bibliography.Springer Nature c/o IBS, Benzstrasse 21, 48619 Heek 168 pp. Englisch.…