Development molecular methods detection di cromeans (23 risultati)
- Brossura
Da: GreatBookPrices, Columbia, MD, U.S.A.GreatBookPrices
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 146,33
EUR 2,27 spedizioneSpedito in U.S.A.Quantità: 10 disponibili
Condizione: New.
- Brossura
Da: Grand Eagle Retail, Bensenville, IL, U.S.A.Grand Eagle Retail
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 148,68
Spedizione gratuitaSpedito in U.S.A.Quantità: 1 disponibili
Paperback. Condizione: new. Paperback. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecu…lar detection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses. A negative sense strand of RNA is generated from the viral positive strand virus, and these two are found primarily bound in a replicative form. This RNA was specifically detected by RT-PCR, including the positive and negative strands. This assay was developed and explored for the detection of low levels of CVB3 and HAV laboratory strains first and then for low levels of wild-type enteroviruses isolated from sewage samples. As few as four infectious units of wild-type enteroviruses, contained in 2.0 ml of water concentrate prepared from 600 ml of treated wastewater effluent, could be detected within two days. Treated wastewater effluents were collected weekly over an 18 months period, and viruses were concentrated with a developed method. About 7% of the final effluent wastewater samples were found positive for infectious enterovirus with the developed RF method, although only 1% of the samples were positive by presence of cytopathic effect. This method avoids the use of two complete cycles of cell culture to detect infectious virus, while confirming infectivity with a molecular method. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.
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Da: PBShop.store US, Wood Dale, IL, U.S.A.PBShop.store US
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 148,79
Spedizione gratuitaSpedito in U.S.A.Quantità: 10 disponibili
PAP. Condizione: New. New Book. Shipped from UK. Established seller since 2000.
- Brossura
Da: PBShop.store UK, Fairford, GLOS, Regno UnitoPBShop.store UK
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 147,15
EUR 3,83 spedizioneSpedito da Regno Unito a U.S.A.Quantità: 10 disponibili
PAP. Condizione: New. New Book. Shipped from UK. Established seller since 2000.
- Brossura
Da: GreatBookPricesUK, Woodford Green, Regno UnitoGreatBookPricesUK
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 147,14
EUR 17,46 spedizioneSpedito da Regno Unito a U.S.A.Quantità: 10 disponibili
Condizione: New.
- Brossura
Da: GreatBookPrices, Columbia, MD, U.S.A.GreatBookPrices
Contatta il venditoreVenditore con 5 stelleCondizione: Usato - Come nuovo
EUR 164,32
EUR 2,27 spedizioneSpedito in U.S.A.Quantità: 10 disponibili
Condizione: As New. Unread book in perfect condition.
- Brossura
Da: Majestic Books, Hounslow, Regno UnitoMajestic Books
Contatta il venditoreVenditore con 4 stelleCondizione: Nuovo
EUR 161,88
EUR 7,56 spedizioneSpedito da Regno Unito a U.S.A.Quantità: 3 disponibili
Condizione: New. pp. 80 Illus.
- Brossura
Da: Books Puddle, New York, NY, U.S.A.Books Puddle
Contatta il venditoreVenditore con 4 stelleCondizione: Nuovo
EUR 171,27
EUR 3,44 spedizioneSpedito in U.S.A.Quantità: 3 disponibili
Condizione: New. pp. 80.
- Brossura
Da: Ria Christie Collections, Uxbridge, Regno UnitoRia Christie Collections
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 161,41
EUR 13,94 spedizioneSpedito da Regno Unito a U.S.A.Quantità: 10 disponibili
Condizione: New. In.
- Brossura
Da: GreatBookPricesUK, Woodford Green, Regno UnitoGreatBookPricesUK
Contatta il venditoreVenditore con 5 stelleCondizione: Usato - Come nuovo
EUR 166,52
EUR 17,46 spedizioneSpedito da Regno Unito a U.S.A.Quantità: 10 disponibili
Condizione: As New. Unread book in perfect condition.
- Brossura
Da: Rarewaves USA, HEBRON, KY, U.S.A.Rarewaves USA
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 186,04
Spedizione gratuitaSpedito in U.S.A.Quantità: 5 disponibili
Paperback. Condizione: New. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detect…ion of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses.
- Brossura
Da: Rarewaves.com USA, London, LONDO, Regno UnitoRarewaves.com USA
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 186,11
Spedizione gratuitaSpedito da Regno Unito a U.S.A.Quantità: 5 disponibili
Paperback. Condizione: New. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detect…ion of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses.
- Brossura
Da: THE SAINT BOOKSTORE, Southport, Regno UnitoTHE SAINT BOOKSTORE
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 172,63
EUR 15,22 spedizioneSpedito da Regno Unito a U.S.A.Quantità: 10 disponibili
Paperback / softback. Condizione: New. New copy - Usually dispatched within 3 working days.
- Brossura
Da: Revaluation Books, Exeter, Regno UnitoRevaluation Books
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 176,98
EUR 11,64 spedizioneSpedito da Regno Unito a U.S.A.Quantità: 2 disponibili
Paperback. Condizione: Brand New. illustrated edition. 78 pages. 10.75x8.25x0.25 inches. In Stock.
- Brossura
Da: Kennys Bookshop and Art Galleries Ltd., Galway, GY, IrlandaKennys Bookshop and Art Galleries Ltd.
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 177,97
EUR 9,50 spedizioneSpedito da Irlanda a U.S.A.Quantità: 10 disponibili
Condizione: New. 2004. paperback. . . . . .
- Brossura
Da: Biblios, frankfurt am main, HESSE, GermaniaBiblios
Contatta il venditoreVenditore con 4 stelleCondizione: Nuovo
EUR 182,94
EUR 9,95 spedizioneSpedito da Germania a U.S.A.Quantità: 3 disponibili
Condizione: New. pp. 80.
- Brossura
Da: moluna, Greven, Germaniamoluna
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 155,63
EUR 48,99 spedizioneSpedito da Germania a U.S.A.Quantità: Più di 20 disponibili
Condizione: New. KlappentextEnteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniq.
- Brossura
Da: Kennys Bookstore, Olney, MD, U.S.A.Kennys Bookstore
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 221,99
EUR 9,04 spedizioneSpedito in U.S.A.Quantità: 10 disponibili
Condizione: New. 2004. paperback. . . . . . Books ship from the US and Ireland.
- Brossura
Da: Rarewaves USA United, HEBRON, KY, U.S.A.Rarewaves USA United
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 188,86
EUR 43,05 spedizioneSpedito in U.S.A.Quantità: 5 disponibili
Paperback. Condizione: New. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detect…ion of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses.
- Brossura
Da: preigu, Osnabrück, Germaniapreigu
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 172,05
EUR 70,00 spedizioneSpedito da Germania a U.S.A.Quantità: 1 disponibili
Taschenbuch. Condizione: Neu. Development of Molecular Methods for Detection of Infectious Viruses in Treated Wastewater | T. Cromeans | Taschenbuch | Werf Research Report | Kartoniert / Broschiert | Englisch | 2005 | Werf | EAN 9781843397229 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 490…78 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu.
- Brossura
Da: AHA-BUCH GmbH, Einbeck, GermaniaAHA-BUCH GmbH
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 183,42
EUR 61,12 spedizioneSpedito da Germania a U.S.A.Quantità: 2 disponibili
Taschenbuch. Condizione: Neu. Neuware - Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molec…ular detection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses. A negative sense strand of RNA is generated from the viral positive strand virus, and these two are found primarily bound in a replicative form. This RNA was specifically detected by RT-PCR, including the positive and negative strands. This assay was developed and explored for the detection of low levels of CVB3 and HAV laboratory strains first and then for low levels of wild-type enteroviruses isolated from sewage samples. As few as four infectious units of wild-type enteroviruses, contained in 2.0 ml of water concentrate prepared from 600 ml of treated wastewater effluent, could be detected within two days. Treated wastewater effluents were collected weekly over an 18 months period, and viruses were concentrated with a developed method. About 7% of the final effluent wastewater samples were found positive for infectious enterovirus with the developed RF method, although only 1% of the samples were positive by presence of cytopathic effect. This method avoids the use of two complete cycles of cell culture to detect infectious virus, while confirming infectivity with a molecular method.
- Brossura
Da: Rarewaves.com UK, London, Regno UnitoRarewaves.com UK
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 181,94
EUR 75,65 spedizioneSpedito da Regno Unito a U.S.A.Quantità: 5 disponibili
Paperback. Condizione: New. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detect…ion of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses.
- Brossura
Da: AussieBookSeller, Truganina, VIC, AustraliaAussieBookSeller
Contatta il venditoreVenditore con 5 stelleCondizione: Nuovo
EUR 245,70
EUR 31,86 spedizioneSpedito da Australia a U.S.A.Quantità: 1 disponibili
Paperback. Condizione: new. Paperback. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecu…lar detection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses. A negative sense strand of RNA is generated from the viral positive strand virus, and these two are found primarily bound in a replicative form. This RNA was specifically detected by RT-PCR, including the positive and negative strands. This assay was developed and explored for the detection of low levels of CVB3 and HAV laboratory strains first and then for low levels of wild-type enteroviruses isolated from sewage samples. As few as four infectious units of wild-type enteroviruses, contained in 2.0 ml of water concentrate prepared from 600 ml of treated wastewater effluent, could be detected within two days. Treated wastewater effluents were collected weekly over an 18 months period, and viruses were concentrated with a developed method. About 7% of the final effluent wastewater samples were found positive for infectious enterovirus with the developed RF method, although only 1% of the samples were positive by presence of cytopathic effect. This method avoids the use of two complete cycles of cell culture to detect infectious virus, while confirming infectivity with a molecular method. Shipping may be from our Sydney, NSW warehouse or from our UK or US warehouse, depending on stock availability.
