Leptospira spp methods protocols (6 risultati)

Taschenbuch. Condizione: Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume looks at all aspects of manipulation of Leptospira spp. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from both clinical and environmental samples; using methods like whole genome sequencing and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry to identify bacterial species; tools for gene inactivation and in vitro and in vivo assays to study the pathogenesis of leptospirosis; and the use of hamsters to evaluate leptospiral virulence and vaccine candidates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Leptospira spp.: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field and these fascinating organisms.…

Condizione: New. 1st ed. 2020 edition NO-PA16APR2015-KAP.

Taschenbuch. Condizione: Neu. Leptospira spp. | Methods and Protocols | Nobuo Koizumi (u. a.) | Taschenbuch | x | Englisch | 2021 | Humana | EAN 9781071604618 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu.…

Condizione: New. Print on Demand.

Condizione: New. PRINT ON DEMAND.

Buch. Condizione: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - This detailed new edition reflects the rapid evolution of Leptospira research over the past decade, particularly in genetics, genomics, imaging, and computational analysis. The book provides practical guidance for the manipulation of Leptospira spp. and for the investigation of leptospiral pathogenesis using state-of-the-art experimental approaches by introducing alternative media for direct isolation from infected hosts and for better mimicking host-associated conditions and by exploring gene editing tools, imaging-based approaches, and host-pathogen interactions, among other subject areas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Leptospira spp.: Methods and Protocols, Second Edition serves both established investigators and newcomers to the field, facilitating rigorous and innovative research on these fastidious yet fascinating organisms.…